- The subject lies on their side, and a standard clinical proctoscope (disposable and included with the kit) is inserted into the rectum (a very common procedure).
- The device is then prepared and inserted into the proctoscope. A syringe is used to inject 80ml of air into the device.
- An elasticated membrane (balloon) is inflated against the rectal wall. The membrane is hydrophilic, attracting a thin layer of the rectal biofilm to its surface.
- After 10 seconds the membrane is retracted (back into the device), and the device removed from the proctoscope, which is then removed and disposed of.
- A stabilisation buffer is added to the sample (contained in a detachable component of the device), which is then sent to the laboratory for analysis.
- The remainder of the device and syringe is then disposed of according to local legislation.