It is generally accepted that physiological renewal of colonocyte populations in the gut results in the exfoliation of millions of epithelial cells each day. Exfoliated colonocytes move within the mucocellular layer separating colonic epithelium from the gut contents {1,2,} down to the rectum, leading to a build-up of exfoliated cells within the rectal mucosa {4}.
Several studies indicate that cell exfoliation in the human colon increases in the presence of localised disease {2-5} such as CRC and IBD. Exfoliated cells from such sites can be found at significant distances from the site of pathology {6}, indicating that exfoliated cells tend to migrate through the colon alongside faecal flow, and ultimately reside in the rectal mucosa.
The Origin Sciences Rectal Cell Sampler (a simple medical device that incorporates an inflatable cell collection membrane) is inserted into the rectal cavity by means of a standard disposable proctoscope. The membrane is deployed and the cellular material is attracted to the surface of the membrane. The membrane is then retracted back into the device and a buffered solution is added to preserve the sample.
The sample is then sent to a dedicated laboratory where the DNA from collected material is purified and quantified. The amount of DNA detected indicates the likelihood of serious colorectal disease being present. The laboratory results are usually returned within 3 days of submission, and are sent directly to the clinician.
A number of papers have been published, which cover the methodology and clinical data. If you would like further information, please contact us
